cytoflowgui.op_plugins.pca

Use principal components analysis (PCA) to decompose a multivariate data set into orthogonal components that explain a maximum amount of variance.

Creates new “channels” named {name}_1 ... {name}_n, where name is the Name attribute and n is Num components.

The same decomposition may not be appropriate for different subsets of the data set. If this is the case, you can use the By attribute to specify metadata by which to aggregate the data before estimating (and applying) a model. The PCA parameters such as the number of components and the kernel are the same across each subset, though.

Name

The operation name; determines the name of the new columns.

Channels

The channels to apply the decomposition to.

Scale

Re-scale the data in the specified channels before fitting.

Num components

How many components to fit to the data? Must be a positive integer.

By

A list of metadata attributes to aggregate the data before estimating the model. For example, if the experiment has two pieces of metadata, Time and Dox, setting By to ["Time", "Dox"] will fit the model separately to each subset of the data with a unique combination of Time and Dox.

Whiten

Scale each component to unit variance? May be useful if you will be using unsupervized clustering (such as K-means).

class cytoflowgui.op_plugins.pca.ChannelHandler(*args: Any, **kwargs: Any)[source]

Bases: traitsui.api.

class cytoflowgui.op_plugins.pca.PCAHandler(*args: Any, **kwargs: Any)[source]

Bases: traitsui.api.

add_channel

alias of traits.trait_types.Event

remove_channel

alias of traits.trait_types.Event

channels = <traits.traits.ForwardProperty object>
class cytoflowgui.op_plugins.pca.PCAPlugin[source]

Bases: envisage.plugin.Plugin, cytoflowgui.op_plugins.op_plugin_base.PluginHelpMixin

operation_id = 'edu.mit.synbio.cytoflow.operations.pca'
view_id = None
short_name = 'Principal Component Analysis'
menu_group = 'Calibration'
get_operation()[source]
get_handler(model, context)[source]
get_icon()[source]