# cytoflowgui.op_plugins.gaussian_2d¶

Fit a Gaussian mixture model with a specified number of components to two channels.

If Num Components is greater than 1, then this module creates a new categorical metadata variable named Name, with possible values `{name}_1` …. `name_n` where `n` is the number of components. An event is assigned to `name_i` category if it has the highest posterior probability of having been produced by component `i`. If an event has a value that is outside the range of one of the channels’ scales, then it is assigned to `{name}_None`.

Additionally, if Sigma is greater than 0, this module creates new boolean metadata variables named `{name}_1``{name}_n` where `n` is the number of components. The column `{name}_i` is `True` if the event is less than Sigma standard deviations from the mean of component `i`. If Num Components is `1`, Sigma must be greater than 0.

Finally, the same mixture model (mean and standard deviation) may not be appropriate for every subset of the data. If this is the case, you can use By to specify metadata by which to aggregate the data before estimating and applying a mixture model.

Note

Num Components and Sigma will be the same for each subset.

Name

The operation name; determines the name of the new metadata

X Channel, Y Channel

The channels to apply the mixture model to.

X Scale, Y Scale

Re-scale the data in Channel before fitting.

Num Components

How many components to fit to the data? Must be a positive integer.

Sigma

How many standard deviations on either side of the mean to include in the boolean variable `{name}_i`? Must be `>= 0.0`. If Num Components is `1`, must be `> 0`.

By

A list of metadata attributes to aggregate the data before estimating the model. For example, if the experiment has two pieces of metadata, `Time` and `Dox`, setting By to `["Time", "Dox"]` will fit the model separately to each subset of the data with a unique combination of `Time` and `Dox`.

class cytoflowgui.op_plugins.gaussian_2d.GaussianMixture2DHandler(*args: Any, **kwargs: Any)[source]
class cytoflowgui.op_plugins.gaussian_2d.GaussianMixture2DViewHandler(*args: Any, **kwargs: Any)[source]
class cytoflowgui.op_plugins.gaussian_2d.GaussianMixture2DPlugin[source]
operation_id = 'edu.mit.synbio.cytoflowgui.operations.gaussian_2d'
view_id = 'edu.mit.synbio.cytoflow.view.gaussianmixture2dview'
short_name = '2D Mixture Model'