Source code for cytoflowgui.view_plugins.stats_1d

#!/usr/bin/env python3.8
# coding: latin-1

# (c) Massachusetts Institute of Technology 2015-2018
# (c) Brian Teague 2018-2022
#
# This program is free software: you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation, either version 2 of the License, or
# (at your option) any later version.
# 
# This program is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
# GNU General Public License for more details.
# 
# You should have received a copy of the GNU General Public License
# along with this program.  If not, see <http://www.gnu.org/licenses/>.

"""
1D Statistics Plot
------------------

Plots a line plot of a statistic.

Each variable in the statistic (ie, each variable chosen in the statistic
operation's **Group By**) must be set as **Variable** or as a facet.

.. object:: Statistic

    Which statistic to plot.
    
.. object:: Feature

    The column in the statistic to plot on the Y axis (often a channel name.)
    
.. object:: Variable

    The statistic variable put on the X axis.  Must be numeric.
    
.. object:: X Scale, Y Scale

    How to scale the X and Y axes.
    
.. object:: Horizontal Facet

    Make muliple plots, with each column representing a subset of the statistic
    with a different value for this variable.
        
.. object:: Vertical Facet

    Make multiple plots, with each row representing a subset of the statistic
    with a different value for this variable.
    
.. object:: Hue Facet

    Make multiple bars with different colors; each color represents a subset
    of the statistic with a different value for this variable.
    
.. object:: Color Scale

    If **Color Facet** is a numeric variable, use this scale for the color
    bar.
    
.. object:: Error Statistic

    A statistic to use to make the error bars.  Must have the same variables
    as the statistic in **Statistic**.
    
.. object:: Subset

    Plot only a subset of the statistic.
    
.. plot::
   :include-source: False

    import cytoflow as flow
    import_op = flow.ImportOp()
    import_op.tubes = [flow.Tube(file = "Plate01/RFP_Well_A3.fcs",
                                 conditions = {'Dox' : 10.0}),
                      flow.Tube(file = "Plate01/CFP_Well_A4.fcs",
                                conditions = {'Dox' : 1.0})]
    import_op.conditions = {'Dox' : 'float'}
    ex = import_op.apply()
    
    ch_op = flow.ChannelStatisticOp(name = 'MeanByDox',
                        channel = 'Y2-A',
                        function = flow.geom_mean,
                        by = ['Dox'])
    ex2 = ch_op.apply(ex)
    
    flow.Stats1DView(variable = 'Dox',
                     statistic = 'MeanByDox',
                     feature = 'Y2-A',
                     scale = 'log',
                     variable_scale = 'log').plot(ex2)
"""

import pandas as pd

from traits.api import provides, Property, List
from traitsui.api import View, Item, EnumEditor, VGroup, TextEditor, Controller, TupleEditor
from envisage.api import Plugin
from pyface.api import ImageResource  # @UnresolvedImport

import cytoflow.utility as util

from ..workflow.views import Stats1DWorkflowView, Stats1DPlotParams
from ..editors import SubsetListEditor, ColorTextEditor, ExtendableEnumEditor, InstanceHandlerEditor
from ..subset_controllers import subset_handler_factory

from .i_view_plugin import IViewPlugin, VIEW_PLUGIN_EXT
from .view_plugin_base import ViewHandler, PluginHelpMixin, Stats1DPlotParamsView


[docs] class Stats1DParamsHandler(Controller): view_params_view = \ View(Item('variable_lim', label = "Variable\nLimits", editor = TupleEditor(editors = [TextEditor(auto_set = False, evaluate = float, format_func = lambda x: "" if x == None else str(x)), TextEditor(auto_set = False, evaluate = float, format_func = lambda x: "" if x == None else str(x))], labels = ["Min", "Max"], cols = 1)), Item('linestyle'), Item('marker'), Item('markersize', editor = TextEditor(auto_set = False), format_func = lambda x: "" if x == None else str(x)), Item('capsize', editor = TextEditor(auto_set = False), format_func = lambda x: "" if x == None else str(x)), Item('alpha'), Item('shade_error'), Item('shade_alpha'), Stats1DPlotParamsView.content)
[docs] class Stats1DHandler(ViewHandler): indices = Property(depends_on = "context.statistics, model.statistic, model.subset") numeric_indices = Property(depends_on = "context.statistics, model.statistic, model.subset") levels = Property(depends_on = "context.statistics, model.statistic") features = Property(depends_on = "context.statistics, model.statistic") view_traits_view = \ View(VGroup( VGroup(Item('statistic', editor=EnumEditor(name='context_handler.statistics_names'), label = "Statistic"), Item('feature', editor = EnumEditor(name='handler.features'), label = "Feature"), Item('scale', label = "Statistic\nScale"), Item('variable', editor = EnumEditor(name = 'handler.numeric_indices')), Item('variable_scale', label = "Variable\nScale"), Item('xfacet', editor=ExtendableEnumEditor(name='handler.indices', extra_items = {"None" : ""}), label = "Horizontal\nFacet"), Item('yfacet', editor=ExtendableEnumEditor(name='handler.indices', extra_items = {"None" : ""}), label = "Vertical\nFacet"), Item('huefacet', editor=ExtendableEnumEditor(name='handler.indices', extra_items = {"None" : ""}), label="Hue\nFacet"), Item('huescale', label = "Hue\nScale"), Item('error_low', editor=ExtendableEnumEditor(name='handler.features', extra_items = {"None" : ""}), label = "Error Bar Low"), Item('error_high', editor=ExtendableEnumEditor(name='handler.features', extra_items = {"None" : ""}), label = "Error Bar High"), label = "One-Dimensional Statistics Plot", show_border = False), VGroup(Item('subset_list', show_label = False, editor = SubsetListEditor(conditions = "handler.levels", editor = InstanceHandlerEditor(view = 'subset_view', handler_factory = subset_handler_factory), mutable = False)), label = "Subset", show_border = False, show_labels = False), Item('context.view_warning', resizable = True, visible_when = 'context.view_warning', editor = ColorTextEditor(foreground_color = "#000000", background_color = "#ffff99")), Item('context.view_error', resizable = True, visible_when = 'context.view_error', editor = ColorTextEditor(foreground_color = "#000000", background_color = "#ff9191")))) view_params_view = \ View(Item('plot_params', editor = InstanceHandlerEditor(view = 'view_params_view', handler_factory = Stats1DParamsHandler), style = 'custom', show_label = False)) # MAGIC: gets the value for the property indices def _get_indices(self): if not (self.context and self.context.statistics and self.model.statistic in self.context.statistics): return [] stat = self.context.statistics[self.model.statistic] data = pd.DataFrame(index = stat.index) if self.model.subset: data = data.query(self.model.subset) if len(data) == 0: return [] names = list(data.index.names) for name in names: unique_values = data.index.get_level_values(name).unique() if len(unique_values) == 1: data.index = data.index.droplevel(name) return list(data.index.names) # MAGIC: gets the value for the property 'levels' # returns a Dict(Str, pd.Series) def _get_levels(self): if not (self.context and self.context.statistics and self.model.statistic in self.context.statistics): return {} stat = self.context.statistics[self.model.statistic] index = stat.index names = list(index.names) for name in names: unique_values = index.get_level_values(name).unique() if len(unique_values) == 1: index = index.droplevel(name) names = list(index.names) ret = {} for name in names: ret[name] = pd.Series(index.get_level_values(name)).sort_values() ret[name] = pd.Series(ret[name].unique()) return ret # MAGIC: gets the value for the property numeric_indices def _get_numeric_indices(self): if not (self.context and self.context.statistics and self.model.statistic in self.context.statistics): return [] stat = self.context.statistics[self.model.statistic] data = pd.DataFrame(index = stat.index) if self.model.subset: data = data.query(self.model.subset) if len(data) == 0: return [] names = list(data.index.names) for name in names: unique_values = data.index.get_level_values(name).unique() if len(unique_values) == 1: data.index = data.index.droplevel(name) data.reset_index(inplace = True) return [x for x in data if util.is_numeric(data[x])] # MAGIC: gets the value for the property "features" def _get_features(self): if not (self.context and self.context.statistics and self.model.statistic in self.context.statistics): return [] stat = self.context.statistics[self.model.statistic] return stat.columns.to_list()
[docs] @provides(IViewPlugin) class Stats1DPlugin(Plugin, PluginHelpMixin): id = 'cytoflowgui.view.stats1d' view_id = 'cytoflow.view.stats1d' name = "1D Statistics View" short_name = "Stats 1D"
[docs] def get_view(self): return Stats1DWorkflowView()
[docs] def get_handler(self, model, context): if isinstance(model, Stats1DWorkflowView): return Stats1DHandler(model = model, context = context) elif isinstance(model, Stats1DPlotParams): return Stats1DParamsHandler(model = model, context = context)
[docs] def get_icon(self): return ImageResource('stats_1d')
plugin = List(contributes_to = VIEW_PLUGIN_EXT) def _plugin_default(self): return [self]