Tutorials

The following are step-by-step examples to get you started using Cytoflow. They include both some basic tutorials as well as examples of some more advanced analyses.

Easy tutorials

• Tutorial: Quickstart
  • Importing data
  • Basic plotting
  • Basic gating
  • Basic statistics
  • Export the plot
• Tutorial: Dose-Response
  • Importing Data
  • Filter out clumps and debris
  • Look at the FITC_A channel
  • Summarize the dose-response curve on a line plot
  • Export the dose-response curve as a table
• Tutorial: Machine Learning
  • Import the data
  • Examine the data
  • Create a Gaussian mixture model
  • Advanced GMM uses
  • More Machine Learning Operations

Advanced tutorials

• Tutorial: Yeast Multidimensional Induction Timecourse
  • Import the data
  • Filter out clumps and debris
  • Compute the geometric mean at each different timepoint and IP concentration
  • Is a geometric mean an appropriate summary statistic?
  • Model the data as a mixture of gaussians
• Tutorial: Synthetic Gene Logic Network
  • Import the data
  • Gate out debris
  • Select transfected cells
  • Examine the function of the gene circuit
  • Further exploration
• Tutorial: Transcriptional Repressor Characterization
  • Setup
  • Gate out the debris
  • TASBE Calibration
  • Binned Analysis
  • Creating Transfer Curves