Source code for cytoflow

#!/usr/bin/env python3.4
# coding: latin-1

# (c) Massachusetts Institute of Technology 2015-2018
# (c) Brian Teague 2018-2021
#
# This program is free software: you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation, either version 2 of the License, or
# (at your option) any later version.
# 
# This program is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
# GNU General Public License for more details.
# 
# You should have received a copy of the GNU General Public License
# along with this program.  If not, see <http://www.gnu.org/licenses/>.

# check python version
import sys
if sys.version_info < (3, 4):
    raise Exception("Cytoflow requires Python 3.4 or later")

# suppress meaningless warnings from seaborn
import warnings
warnings.filterwarnings('ignore', '.*IPython widgets are experimental.*')
warnings.filterwarnings('ignore', 'axes.color_cycle is deprecated and replaced with axes.prop_cycle')

# and matplotlib 3.1.1 -- there's some weird interaction with seaborn here.
import matplotlib.text
import logging
[docs]class MplFilter(logging.Filter):
[docs] def filter(self, record): if record.msg == "posx and posy should be finite values": return 0 else: return 1
matplotlib.text._log.addFilter(MplFilter()) # keep track of whether we're running in the GUI. # there is the occasional place where we differ in behavior RUNNING_IN_GUI = False # basics from .experiment import Experiment from .operations.import_op import ImportOp, Tube # gates from .operations.threshold import ThresholdOp from .operations.range import RangeOp from .operations.range2d import Range2DOp from .operations.polygon import PolygonOp from .operations.quad import QuadOp # TASBE from .operations.autofluorescence import AutofluorescenceOp from .operations.bleedthrough_piecewise import BleedthroughPiecewiseOp from .operations.bleedthrough_linear import BleedthroughLinearOp from .operations.bead_calibration import BeadCalibrationOp from .operations.color_translation import ColorTranslationOp # data-driven from .operations.ratio import RatioOp from .operations.density import DensityGateOp from .operations.gaussian import GaussianMixtureOp from .operations.kmeans import KMeansOp from .operations.flowpeaks import FlowPeaksOp from .operations.pca import PCAOp # channels from .operations.channel_stat import ChannelStatisticOp from .operations.frame_stat import FrameStatisticOp from .operations.xform_stat import TransformStatisticOp # misc from .operations.binning import BinningOp # views from .views.histogram import HistogramView from .views.scatterplot import ScatterplotView from .views.densityplot import DensityView from .views.stats_1d import Stats1DView from .views.stats_2d import Stats2DView from .views.bar_chart import BarChartView from .views.kde_1d import Kde1DView from .views.kde_2d import Kde2DView from .views.histogram_2d import Histogram2DView from .views.violin import ViolinPlotView from .views.table import TableView from .views.radviz import RadvizView from .views.parallel_coords import ParallelCoordinatesView from .views.export_fcs import ExportFCS # util from .utility.util_functions import (geom_mean, geom_sd, geom_sd_range, geom_sem, geom_sem_range) from .utility.algorithms import (ci, percentiles) from .utility.scale import set_default_scale, get_default_scale from ._version import get_versions # @UnresolvedImport __version__ = get_versions()['version'] del get_versions